FastQCFastQC Report
Sun 7 Feb 2021
zr3616_7_R1_val_1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr3616_7_R1_val_1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149080064
Sequences flagged as poor quality0
Sequence length20-115
%GC19

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAATCGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGGG5725540.38405805889645983Illumina Paired End PCR Primer 2 (96% over 27bp)
GACCAATCGGCTGATCGCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGGG2074800.13917353832099238Illumina PCR Primer Index 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)