FastQCFastQC Report
Sun 7 Feb 2021
zr3616_5_R1_val_1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr3616_5_R1_val_1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148556552
Sequences flagged as poor quality0
Sequence length20-115
%GC19

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTGTAATCGACATCTCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGGG8023170.5400751358311009RNA PCR Primer, Index 41 (96% over 29bp)
GACTGTAATCGACATCGCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGGG3431780.23100832334880791Illumina PCR Primer Index 11 (96% over 26bp)
TGTAATCGACATCTCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGGGGGG1543900.10392675241950958RNA PCR Primer, Index 41 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)