FastQCFastQC Report
Sun 7 Feb 2021
zr3616_3_R1_val_1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr3616_3_R1_val_1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188375666
Sequences flagged as poor quality0
Sequence length20-115
%GC20

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGGTAACTCGCATCTCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGGG14216530.7546903643063961TruSeq Adapter, Index 18 (96% over 28bp)
GACGGTAACTCGCATCGCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGGG4831970.25650712231589406Illumina PCR Primer Index 3 (96% over 26bp)
GGTAACTCGCATCTCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGGGGGG2570250.13644278237083976TruSeq Adapter, Index 18 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)