FastQCFastQC Report
Tue 2 Feb 2021
zr3616_6_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr3616_6_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197572800
Sequences flagged as poor quality0
Sequence length20-135
%GC20

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGTGCAGACAGATCTCGTATGCCGTCTTCTGCTTGAAA14266640.7220953491573738Illumina Paired End PCR Primer 2 (96% over 30bp)
GAGCCCACGAGACGTGCAGACAGATCGCGTATGCCGTCTTCTGCTTGAAA4771960.24152919835119002Illumina PCR Primer Index 9 (96% over 27bp)
CCCACGAGACGTGCAGACAGATCTCGTATGCCGTCTTCTGCTTGAAAAGG2475580.12529963638719502Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)