FastQCFastQC Report
Tue 2 Feb 2021
zr3616_5_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr3616_5_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150419008
Sequences flagged as poor quality0
Sequence length20-135
%GC19

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACTGTAATCGACATCTCGTATGCCGTCTTCTGCTTGAAA9098600.6048836593843246TruSeq Adapter, Index 11 (96% over 28bp)
GAGCCCACGAGACTGTAATCGACATCGCGTATGCCGTCTTCTGCTTGAAA3820470.25398851187743504RNA PCR Primer, Index 41 (96% over 28bp)
CCCACGAGACTGTAATCGACATCTCGTATGCCGTCTTCTGCTTGAAAAGG1545860.10277025626973953TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)