FastQCFastQC Report
Tue 2 Feb 2021
zr3616_1_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr3616_1_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162780101
Sequences flagged as poor quality0
Sequence length20-135
%GC20

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGCCGCACTCTATCTCGTATGCCGTCTTCTGCTTGAAA11802610.7250646686845341RNA PCR Primer, Index 32 (96% over 30bp)
GAGCCCACGAGACGCCGCACTCTATCGCGTATGCCGTCTTCTGCTTGAAA4388140.269574719086825RNA PCR Primer, Index 27 (96% over 26bp)
CCCACGAGACGCCGCACTCTATCTCGTATGCCGTCTTCTGCTTGAAAAGG1810200.11120523877792654RNA PCR Primer, Index 32 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)