Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_8_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_8_R2_val_2_val_2_val_2.fq.gz

Data processed at 00:59:55 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 159382736
Paired-end alignments with a unique best hit 99905373
Pairs without alignments under any condition 35222170
Pairs that did not map uniquely 24255193
Genomic sequence context not extractable (edges of chromosomes) 10942

Cytosine Methylation

Total C's analysed 3278700792
Methylated C's in CpG context 49286084
Methylated C's in CHG context 3189822
Methylated C's in CHH context 13482993
Methylated C's in Unknown context 135498
Unmethylated C's in CpG context 388610874
Unmethylated C's in CHG context 591662815
Unmethylated C's in CHH context 2232468204
Unmethylated C's in Unknown context 7848334
Percentage methylation (CpG context) 11.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 25050830 original top strand
CTOT 24924272 complementary to original top strand
CTOB 24899415 complementary to original bottom strand
OB 25019914 original bottom strand

Deduplication

Alignments analysed 99894431
Unique alignments 76147582
Duplicates removed 23746849
Duplicated alignments were found at 17756204 different positions

Cytosine Methylation after Extraction

Total C's analysed 1487174194
Methylated C's in CpG context 21924067
Methylated C's in CHG context 1433325
Methylated C's in CHH context 6168440
Unmethylated C's in CpG context 174580678
Unmethylated C's in CHG context 265499317
Unmethylated C's in CHH context 1017568367
Percentage methylation (CpG context) 11.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot