Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_7_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_7_R2_val_2_val_2_val_2.fq.gz

Data processed at 00:59:55 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 147518415
Paired-end alignments with a unique best hit 92397854
Pairs without alignments under any condition 33293781
Pairs that did not map uniquely 21826780
Genomic sequence context not extractable (edges of chromosomes) 8730

Cytosine Methylation

Total C's analysed 2987092697
Methylated C's in CpG context 46401363
Methylated C's in CHG context 2864285
Methylated C's in CHH context 12367749
Methylated C's in Unknown context 131339
Unmethylated C's in CpG context 348991413
Unmethylated C's in CHG context 536115981
Unmethylated C's in CHH context 2040351906
Unmethylated C's in Unknown context 7221933
Percentage methylation (CpG context) 11.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 23238625 original top strand
CTOT 22873446 complementary to original top strand
CTOB 22955941 complementary to original bottom strand
OB 23321112 original bottom strand

Deduplication

Alignments analysed 92389124
Unique alignments 71112087
Duplicates removed 21277037
Duplicated alignments were found at 16017094 different positions

Cytosine Methylation after Extraction

Total C's analysed 1370876734
Methylated C's in CpG context 20893662
Methylated C's in CHG context 1301565
Methylated C's in CHH context 5719406
Unmethylated C's in CpG context 158626227
Unmethylated C's in CHG context 243508676
Unmethylated C's in CHH context 940827198
Percentage methylation (CpG context) 11.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot