Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_6_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_6_R2_val_2_val_2_val_2.fq.gz

Data processed at 00:59:54 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 191560039
Paired-end alignments with a unique best hit 119684660
Pairs without alignments under any condition 41837372
Pairs that did not map uniquely 30038007
Genomic sequence context not extractable (edges of chromosomes) 12058

Cytosine Methylation

Total C's analysed 3820668638
Methylated C's in CpG context 56568946
Methylated C's in CHG context 3598036
Methylated C's in CHH context 15351455
Methylated C's in Unknown context 160518
Unmethylated C's in CpG context 454902768
Unmethylated C's in CHG context 690584257
Unmethylated C's in CHH context 2599663176
Unmethylated C's in Unknown context 9120689
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 29988260 original top strand
CTOT 29768713 complementary to original top strand
CTOB 29852231 complementary to original bottom strand
OB 30063398 original bottom strand

Deduplication

Alignments analysed 119672602
Unique alignments 87439346
Duplicates removed 32233256
Duplicated alignments were found at 23154708 different positions

Cytosine Methylation after Extraction

Total C's analysed 1612993703
Methylated C's in CpG context 23485488
Methylated C's in CHG context 1508024
Methylated C's in CHH context 6600387
Unmethylated C's in CpG context 190223768
Unmethylated C's in CHG context 288713351
Unmethylated C's in CHH context 1102462685
Percentage methylation (CpG context) 11.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot