Bismark report for: /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_5_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_5_R2_val_2_val_2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/yaaminiv/Manchester/data/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 146393061 Number of paired-end alignments with a unique best hit: 91751559 Mapping efficiency: 62.7% Sequence pairs with no alignments under any condition: 32179257 Sequence pairs did not map uniquely: 22462245 Sequence pairs which were discarded because genomic sequence could not be extracted: 9714 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 22931856 ((converted) top strand) GA/CT/CT: 22891689 (complementary to (converted) top strand) GA/CT/GA: 22940535 (complementary to (converted) bottom strand) CT/GA/GA: 22977765 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2984206921 Total methylated C's in CpG context: 42797776 Total methylated C's in CHG context: 2791881 Total methylated C's in CHH context: 12059840 Total methylated C's in Unknown context: 121647 Total unmethylated C's in CpG context: 354838511 Total unmethylated C's in CHG context: 537759878 Total unmethylated C's in CHH context: 2033959035 Total unmethylated C's in Unknown context: 7141330 C methylated in CpG context: 10.8% C methylated in CHG context: 0.5% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 1.7% Bismark completed in 0d 13h 50m 57s