Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_5_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_5_R2_val_2_val_2_val_2.fq.gz

Data processed at 00:59:54 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 146393061
Paired-end alignments with a unique best hit 91751559
Pairs without alignments under any condition 32179257
Pairs that did not map uniquely 22462245
Genomic sequence context not extractable (edges of chromosomes) 9714

Cytosine Methylation

Total C's analysed 2984206921
Methylated C's in CpG context 42797776
Methylated C's in CHG context 2791881
Methylated C's in CHH context 12059840
Methylated C's in Unknown context 121647
Unmethylated C's in CpG context 354838511
Unmethylated C's in CHG context 537759878
Unmethylated C's in CHH context 2033959035
Unmethylated C's in Unknown context 7141330
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 22931856 original top strand
CTOT 22891689 complementary to original top strand
CTOB 22940535 complementary to original bottom strand
OB 22977765 original bottom strand

Deduplication

Alignments analysed 91741845
Unique alignments 70580872
Duplicates removed 21160973
Duplicated alignments were found at 15829462 different positions

Cytosine Methylation after Extraction

Total C's analysed 1345267959
Methylated C's in CpG context 18961603
Methylated C's in CHG context 1249297
Methylated C's in CHH context 5492379
Unmethylated C's in CpG context 158137824
Unmethylated C's in CHG context 240045901
Unmethylated C's in CHH context 921380955
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot