Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_4_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_4_R2_val_2_val_2_val_2.fq.gz

Data processed at 00:59:54 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 195404524
Paired-end alignments with a unique best hit 122456913
Pairs without alignments under any condition 43400673
Pairs that did not map uniquely 29546938
Genomic sequence context not extractable (edges of chromosomes) 12448

Cytosine Methylation

Total C's analysed 4026686552
Methylated C's in CpG context 61517114
Methylated C's in CHG context 3775942
Methylated C's in CHH context 15774060
Methylated C's in Unknown context 162452
Unmethylated C's in CpG context 476713646
Unmethylated C's in CHG context 729091776
Unmethylated C's in CHH context 2739814014
Unmethylated C's in Unknown context 9663311
Percentage methylation (CpG context) 11.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 30724486 original top strand
CTOT 30515064 complementary to original top strand
CTOB 30491942 complementary to original bottom strand
OB 30712973 original bottom strand

Deduplication

Alignments analysed 122444465
Unique alignments 91149672
Duplicates removed 31294793
Duplicated alignments were found at 22894892 different positions

Cytosine Methylation after Extraction

Total C's analysed 1779281695
Methylated C's in CpG context 26627845
Methylated C's in CHG context 1652985
Methylated C's in CHH context 7054416
Unmethylated C's in CpG context 208499323
Unmethylated C's in CHG context 318667466
Unmethylated C's in CHH context 1216779660
Percentage methylation (CpG context) 11.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot