Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_3_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_3_R2_val_2_val_2_val_2.fq.gz

Data processed at 00:59:54 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 184625873
Paired-end alignments with a unique best hit 115959769
Pairs without alignments under any condition 40261338
Pairs that did not map uniquely 28404766
Genomic sequence context not extractable (edges of chromosomes) 11248

Cytosine Methylation

Total C's analysed 3698776401
Methylated C's in CpG context 55232294
Methylated C's in CHG context 3406696
Methylated C's in CHH context 14523371
Methylated C's in Unknown context 153488
Unmethylated C's in CpG context 433812436
Unmethylated C's in CHG context 664656714
Unmethylated C's in CHH context 2527144890
Unmethylated C's in Unknown context 8861712
Percentage methylation (CpG context) 11.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 29066834 original top strand
CTOT 28943533 complementary to original top strand
CTOB 28910221 complementary to original bottom strand
OB 29027933 original bottom strand

Deduplication

Alignments analysed 115948521
Unique alignments 85875946
Duplicates removed 30072575
Duplicated alignments were found at 21859669 different positions

Cytosine Methylation after Extraction

Total C's analysed 1587542268
Methylated C's in CpG context 23273152
Methylated C's in CHG context 1449594
Methylated C's in CHH context 6348955
Unmethylated C's in CpG context 185004883
Unmethylated C's in CHG context 282666375
Unmethylated C's in CHH context 1088799309
Percentage methylation (CpG context) 11.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot