Bismark report for: /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_2_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_2_R2_val_2_val_2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/yaaminiv/Manchester/data/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 164737166 Number of paired-end alignments with a unique best hit: 103087584 Mapping efficiency: 62.6% Sequence pairs with no alignments under any condition: 37247825 Sequence pairs did not map uniquely: 24401757 Sequence pairs which were discarded because genomic sequence could not be extracted: 11462 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 25819132 ((converted) top strand) GA/CT/CT: 25684248 (complementary to (converted) top strand) GA/CT/GA: 25715010 (complementary to (converted) bottom strand) CT/GA/GA: 25857732 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3444022931 Total methylated C's in CpG context: 50751840 Total methylated C's in CHG context: 3314562 Total methylated C's in CHH context: 13873691 Total methylated C's in Unknown context: 137554 Total unmethylated C's in CpG context: 412119007 Total unmethylated C's in CHG context: 621569062 Total unmethylated C's in CHH context: 2342394769 Total unmethylated C's in Unknown context: 8332795 C methylated in CpG context: 11.0% C methylated in CHG context: 0.5% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 1.6% Bismark completed in 0d 15h 50m 47s