Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_2_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_2_R2_val_2_val_2_val_2.fq.gz

Data processed at 00:59:54 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 164737166
Paired-end alignments with a unique best hit 103087584
Pairs without alignments under any condition 37247825
Pairs that did not map uniquely 24401757
Genomic sequence context not extractable (edges of chromosomes) 11462

Cytosine Methylation

Total C's analysed 3444022931
Methylated C's in CpG context 50751840
Methylated C's in CHG context 3314562
Methylated C's in CHH context 13873691
Methylated C's in Unknown context 137554
Unmethylated C's in CpG context 412119007
Unmethylated C's in CHG context 621569062
Unmethylated C's in CHH context 2342394769
Unmethylated C's in Unknown context 8332795
Percentage methylation (CpG context) 11.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 25819132 original top strand
CTOT 25684248 complementary to original top strand
CTOB 25715010 complementary to original bottom strand
OB 25857732 original bottom strand

Deduplication

Alignments analysed 103076122
Unique alignments 77324772
Duplicates removed 25751350
Duplicated alignments were found at 18937302 different positions

Cytosine Methylation after Extraction

Total C's analysed 1556124219
Methylated C's in CpG context 22388639
Methylated C's in CHG context 1477588
Methylated C's in CHH context 6334575
Unmethylated C's in CpG context 184146364
Unmethylated C's in CHG context 277529881
Unmethylated C's in CHH context 1064247172
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot