Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_1_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_1_R2_val_2_val_2_val_2.fq.gz

Data processed at 00:59:54 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 158456630
Paired-end alignments with a unique best hit 100061777
Pairs without alignments under any condition 34906989
Pairs that did not map uniquely 23487864
Genomic sequence context not extractable (edges of chromosomes) 11067

Cytosine Methylation

Total C's analysed 3360381662
Methylated C's in CpG context 50113396
Methylated C's in CHG context 3198912
Methylated C's in CHH context 13552022
Methylated C's in Unknown context 135286
Unmethylated C's in CpG context 403993682
Unmethylated C's in CHG context 608483348
Unmethylated C's in CHH context 2281040302
Unmethylated C's in Unknown context 8058665
Percentage methylation (CpG context) 11.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 24950391 original top strand
CTOT 25071053 complementary to original top strand
CTOB 25068429 complementary to original bottom strand
OB 24960837 original bottom strand

Deduplication

Alignments analysed 100050710
Unique alignments 75587481
Duplicates removed 24463229
Duplicated alignments were found at 18108957 different positions

Cytosine Methylation after Extraction

Total C's analysed 1535805923
Methylated C's in CpG context 22355264
Methylated C's in CHG context 1443507
Methylated C's in CHH context 6257301
Unmethylated C's in CpG context 182468128
Unmethylated C's in CHG context 274799054
Unmethylated C's in CHH context 1048482669
Percentage methylation (CpG context) 10.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot