Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_7_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_7_R2_val_2_val_2_val_2.fq.gz

Data processed at 02:09:07 on 2021-03-22


Alignment Stats

Sequence pairs analysed in total 147518415
Paired-end alignments with a unique best hit 91034855
Pairs without alignments under any condition 28400781
Pairs that did not map uniquely 28082779
Genomic sequence context not extractable (edges of chromosomes) 480

Cytosine Methylation

Total C's analysed 2954641746
Methylated C's in CpG context 45632004
Methylated C's in CHG context 2798783
Methylated C's in CHH context 11876787
Methylated C's in Unknown context 128658
Unmethylated C's in CpG context 352122839
Unmethylated C's in CHG context 529254237
Unmethylated C's in CHH context 2012957096
Unmethylated C's in Unknown context 6961644
Percentage methylation (CpG context) 11.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 22981945 original top strand
CTOT 22622460 complementary to original top strand
CTOB 22527011 complementary to original bottom strand
OB 22902959 original bottom strand

Deduplication

Alignments analysed 91034375
Unique alignments 70450786
Duplicates removed 20583589
Duplicated alignments were found at 15677434 different positions

Cytosine Methylation after Extraction

Total C's analysed 1365975623
Methylated C's in CpG context 20679742
Methylated C's in CHG context 1281705
Methylated C's in CHH context 5585942
Unmethylated C's in CpG context 159721711
Unmethylated C's in CHG context 241903748
Unmethylated C's in CHH context 936802775
Percentage methylation (CpG context) 11.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot