Bismark report for: /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_5_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_5_R2_val_2_val_2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/yaaminiv/Hawes/data/Cg-roslin/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 146393061 Number of paired-end alignments with a unique best hit: 90252987 Mapping efficiency: 61.7% Sequence pairs with no alignments under any condition: 27270882 Sequence pairs did not map uniquely: 28869192 Sequence pairs which were discarded because genomic sequence could not be extracted: 323 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 22574512 ((converted) top strand) GA/CT/CT: 22529452 (complementary to (converted) top strand) GA/CT/GA: 22556151 (complementary to (converted) bottom strand) CT/GA/GA: 22592549 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2949353611 Total methylated C's in CpG context: 42197405 Total methylated C's in CHG context: 2737128 Total methylated C's in CHH context: 11517755 Total methylated C's in Unknown context: 118374 Total unmethylated C's in CpG context: 358332480 Total unmethylated C's in CHG context: 530661176 Total unmethylated C's in CHH context: 2003907667 Total unmethylated C's in Unknown context: 6879487 C methylated in CpG context: 10.5% C methylated in CHG context: 0.5% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 1.7% Bismark completed in 0d 13h 44m 21s