Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_5_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_5_R2_val_2_val_2_val_2.fq.gz

Data processed at 02:09:07 on 2021-03-22


Alignment Stats

Sequence pairs analysed in total 146393061
Paired-end alignments with a unique best hit 90252987
Pairs without alignments under any condition 27270882
Pairs that did not map uniquely 28869192
Genomic sequence context not extractable (edges of chromosomes) 323

Cytosine Methylation

Total C's analysed 2949353611
Methylated C's in CpG context 42197405
Methylated C's in CHG context 2737128
Methylated C's in CHH context 11517755
Methylated C's in Unknown context 118374
Unmethylated C's in CpG context 358332480
Unmethylated C's in CHG context 530661176
Unmethylated C's in CHH context 2003907667
Unmethylated C's in Unknown context 6879487
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 22574512 original top strand
CTOT 22529452 complementary to original top strand
CTOB 22556151 complementary to original bottom strand
OB 22592549 original bottom strand

Deduplication

Alignments analysed 90252664
Unique alignments 69896131
Duplicates removed 20356533
Duplicated alignments were found at 15466255 different positions

Cytosine Methylation after Extraction

Total C's analysed 1340317579
Methylated C's in CpG context 18817368
Methylated C's in CHG context 1235977
Methylated C's in CHH context 5346971
Unmethylated C's in CpG context 159399494
Unmethylated C's in CHG context 238482390
Unmethylated C's in CHH context 917035379
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot