Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_4_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_4_R2_val_2_val_2_val_2.fq.gz

Data processed at 02:09:06 on 2021-03-22


Alignment Stats

Sequence pairs analysed in total 195404524
Paired-end alignments with a unique best hit 120720568
Pairs without alignments under any condition 36680613
Pairs that did not map uniquely 38003343
Genomic sequence context not extractable (edges of chromosomes) 496

Cytosine Methylation

Total C's analysed 3990533393
Methylated C's in CpG context 60367388
Methylated C's in CHG context 3685754
Methylated C's in CHH context 15262451
Methylated C's in Unknown context 160090
Unmethylated C's in CpG context 484457471
Unmethylated C's in CHG context 721350336
Unmethylated C's in CHH context 2705409993
Unmethylated C's in Unknown context 9285240
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 30293051 original top strand
CTOT 30082152 complementary to original top strand
CTOB 30060417 complementary to original bottom strand
OB 30284452 original bottom strand

Deduplication

Alignments analysed 120720072
Unique alignments 90381383
Duplicates removed 30338689
Duplicated alignments were found at 22405429 different positions

Cytosine Methylation after Extraction

Total C's analysed 1774937848
Methylated C's in CpG context 26332786
Methylated C's in CHG context 1627724
Methylated C's in CHH context 6917739
Unmethylated C's in CpG context 210401729
Unmethylated C's in CHG context 316973218
Unmethylated C's in CHH context 1212684652
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot