Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_3_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_3_R2_val_2_val_2_val_2.fq.gz

Data processed at 02:09:06 on 2021-03-22


Alignment Stats

Sequence pairs analysed in total 184625873
Paired-end alignments with a unique best hit 113619362
Pairs without alignments under any condition 34374786
Pairs that did not map uniquely 36631725
Genomic sequence context not extractable (edges of chromosomes) 385

Cytosine Methylation

Total C's analysed 3641280967
Methylated C's in CpG context 54179448
Methylated C's in CHG context 3329156
Methylated C's in CHH context 13990966
Methylated C's in Unknown context 147938
Unmethylated C's in CpG context 436869326
Unmethylated C's in CHG context 653627897
Unmethylated C's in CHH context 2479284174
Unmethylated C's in Unknown context 8508477
Percentage methylation (CpG context) 11.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 28443731 original top strand
CTOT 28325206 complementary to original top strand
CTOB 28359599 complementary to original bottom strand
OB 28490441 original bottom strand

Deduplication

Alignments analysed 113618977
Unique alignments 84790314
Duplicates removed 28828663
Duplicated alignments were found at 21300686 different positions

Cytosine Methylation after Extraction

Total C's analysed 1575986604
Methylated C's in CpG context 23032054
Methylated C's in CHG context 1430950
Methylated C's in CHH context 6185606
Unmethylated C's in CpG context 185352566
Unmethylated C's in CHG context 279856496
Unmethylated C's in CHH context 1080128932
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot