Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_2_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_2_R2_val_2_val_2_val_2.fq.gz

Data processed at 02:09:06 on 2021-03-22


Alignment Stats

Sequence pairs analysed in total 164737166
Paired-end alignments with a unique best hit 101710771
Pairs without alignments under any condition 31882668
Pairs that did not map uniquely 31143727
Genomic sequence context not extractable (edges of chromosomes) 314

Cytosine Methylation

Total C's analysed 3417738104
Methylated C's in CpG context 50246857
Methylated C's in CHG context 3247270
Methylated C's in CHH context 13382287
Methylated C's in Unknown context 135691
Unmethylated C's in CpG context 419182139
Unmethylated C's in CHG context 616541822
Unmethylated C's in CHH context 2315137729
Unmethylated C's in Unknown context 8087053
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 25459242 original top strand
CTOT 25336051 complementary to original top strand
CTOB 25377276 complementary to original bottom strand
OB 25537888 original bottom strand

Deduplication

Alignments analysed 101710457
Unique alignments 76654951
Duplicates removed 25055506
Duplicated alignments were found at 18556896 different positions

Cytosine Methylation after Extraction

Total C's analysed 1552669056
Methylated C's in CpG context 22304950
Methylated C's in CHG context 1458775
Methylated C's in CHH context 6198029
Unmethylated C's in CpG context 185654989
Unmethylated C's in CHG context 276338681
Unmethylated C's in CHH context 1060713632
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot