Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_1_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Manchester/analyses/trimgalore-2/poly-G/zr3616_1_R2_val_2_val_2_val_2.fq.gz

Data processed at 02:09:06 on 2021-03-22


Alignment Stats

Sequence pairs analysed in total 158456630
Paired-end alignments with a unique best hit 98315254
Pairs without alignments under any condition 29699917
Pairs that did not map uniquely 30441459
Genomic sequence context not extractable (edges of chromosomes) 408

Cytosine Methylation

Total C's analysed 3319534389
Methylated C's in CpG context 49312443
Methylated C's in CHG context 3125143
Methylated C's in CHH context 12997263
Methylated C's in Unknown context 131492
Unmethylated C's in CpG context 409004180
Unmethylated C's in CHG context 600629677
Unmethylated C's in CHH context 2244465683
Unmethylated C's in Unknown context 7790466
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 24530010 original top strand
CTOT 24630957 complementary to original top strand
CTOB 24634899 complementary to original bottom strand
OB 24518980 original bottom strand

Deduplication

Alignments analysed 98314846
Unique alignments 74780342
Duplicates removed 23534504
Duplicated alignments were found at 17674069 different positions

Cytosine Methylation after Extraction

Total C's analysed 1528910858
Methylated C's in CpG context 22178795
Methylated C's in CHG context 1425230
Methylated C's in CHH context 6104487
Unmethylated C's in CpG context 183605703
Unmethylated C's in CHG context 272920546
Unmethylated C's in CHH context 1042676097
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.6%

M-Bias Plot