Bismark

Bismark Processing Report

/gscratch/scrubbed/yaamini/data/Gigas-WGBS/2019-09-03-Trimmed-Files/YRVL_R1_001.fastq.gz and /gscratch/scrubbed/yaamini/data/Gigas-WGBS/2019-09-03-Trimmed-Files/YRVL_R2_001.fastq.gz

Data processed at 23:10 on 2020-02-12


Alignment Stats

Sequence pairs analysed in total 66590575
Paired-end alignments with a unique best hit 42554384
Pairs without alignments under any condition 19593685
Pairs that did not map uniquely 4442506
Genomic sequence context not extractable (edges of chromosomes) 15181

Cytosine Methylation

Total C's analysed 1975323986
Methylated C's in CpG context 36708077
Methylated C's in CHG context 12605227
Methylated C's in CHH context 59188038
Methylated C's in Unknown context 2509847
Unmethylated C's in CpG context 203868749
Unmethylated C's in CHG context 314647529
Unmethylated C's in CHH context 1348306366
Unmethylated C's in Unknown context 12087334
Percentage methylation (CpG context) 15.3%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 4.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 21265268 original top strand
CTOT 12105 complementary to original top strand
CTOB 13209 complementary to original bottom strand
OB 21248621 original bottom strand

Deduplication

Alignments analysed 42539203
Unique alignments 38688147
Duplicates removed 3851056
Duplicated alignments were found at 3464155 different positions

Cytosine Methylation after Extraction

Total C's analysed 1392804462
Methylated C's in CpG context 26308437
Methylated C's in CHG context 9550930
Methylated C's in CHH context 44530329
Unmethylated C's in CpG context 143696342
Unmethylated C's in CHG context 221362266
Unmethylated C's in CHH context 947356158
Percentage methylation (CpG context) 15.5%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 4.5%

M-Bias Plot