Bismark

Bismark Processing Report

/gscratch/scrubbed/yaamini/data/Gigas-WGBS/2019-09-03-Trimmed-Files/YRVA_R1_001.fastq.gz and /gscratch/scrubbed/yaamini/data/Gigas-WGBS/2019-09-03-Trimmed-Files/YRVA_R2_001.fastq.gz

Data processed at 23:10 on 2020-02-12


Alignment Stats

Sequence pairs analysed in total 67542867
Paired-end alignments with a unique best hit 43217945
Pairs without alignments under any condition 19815721
Pairs that did not map uniquely 4509201
Genomic sequence context not extractable (edges of chromosomes) 15910

Cytosine Methylation

Total C's analysed 2014476985
Methylated C's in CpG context 38388669
Methylated C's in CHG context 12919248
Methylated C's in CHH context 61221106
Methylated C's in Unknown context 2748438
Unmethylated C's in CpG context 208388236
Unmethylated C's in CHG context 321769730
Unmethylated C's in CHH context 1371789996
Unmethylated C's in Unknown context 12474356
Percentage methylation (CpG context) 15.6%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 4.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 21607927 original top strand
CTOT 16626 complementary to original top strand
CTOB 17271 complementary to original bottom strand
OB 21560211 original bottom strand

Deduplication

Alignments analysed 43202035
Unique alignments 38329181
Duplicates removed 4872854
Duplicated alignments were found at 4302912 different positions

Cytosine Methylation after Extraction

Total C's analysed 1373661079
Methylated C's in CpG context 26565256
Methylated C's in CHG context 9456529
Methylated C's in CHH context 44402726
Unmethylated C's in CpG context 141927641
Unmethylated C's in CHG context 218892506
Unmethylated C's in CHH context 932416421
Percentage methylation (CpG context) 15.8%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 4.5%

M-Bias Plot