Bismark report for: YRVL_R1_001.fastq.gz and YRVL_R2_001.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-gigas-oa-meth/analyses/2019-08-30-Bismark-Parameter-Testing/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 6946 Mapping efficiency: 69.5% Sequence pairs with no alignments under any condition: 2336 Sequence pairs did not map uniquely: 718 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3475 ((converted) top strand) GA/CT/CT: 3 (complementary to (converted) top strand) GA/CT/GA: 7 (complementary to (converted) bottom strand) CT/GA/GA: 3460 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 317202 Total methylated C's in CpG context: 5940 Total methylated C's in CHG context: 2044 Total methylated C's in CHH context: 9978 Total methylated C's in Unknown context: 448 Total unmethylated C's in CpG context: 31832 Total unmethylated C's in CHG context: 49968 Total unmethylated C's in CHH context: 217440 Total unmethylated C's in Unknown context: 2578 C methylated in CpG context: 15.7% C methylated in CHG context: 3.9% C methylated in CHH context: 4.4% C methylated in unknown context (CN or CHN): 14.8% Bismark completed in 0d 0h 0m 31s