Bismark report for: YRVA_R1_001.fastq.gz and YRVA_R2_001.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-gigas-oa-meth/analyses/2019-08-30-Bismark-Parameter-Testing/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 6843 Mapping efficiency: 68.4% Sequence pairs with no alignments under any condition: 2483 Sequence pairs did not map uniquely: 674 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3401 ((converted) top strand) GA/CT/CT: 7 (complementary to (converted) top strand) GA/CT/GA: 9 (complementary to (converted) bottom strand) CT/GA/GA: 3424 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 312798 Total methylated C's in CpG context: 6087 Total methylated C's in CHG context: 1989 Total methylated C's in CHH context: 10054 Total methylated C's in Unknown context: 569 Total unmethylated C's in CpG context: 32466 Total unmethylated C's in CHG context: 50013 Total unmethylated C's in CHH context: 212189 Total unmethylated C's in Unknown context: 2463 C methylated in CpG context: 15.8% C methylated in CHG context: 3.8% C methylated in CHH context: 4.5% C methylated in unknown context (CN or CHN): 18.8% Bismark completed in 0d 0h 0m 30s