Bismark report for: /gscratch/scrubbed/yaamini/data/Virginica-MBD/2018-10-17-Trimmed-Files//zr2096_5_s1_R1_val_1.fq.gz and /gscratch/scrubbed/yaamini/data/Virginica-MBD/2018-10-17-Trimmed-Files//zr2096_5_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/yaamini/data/Virginica-MBD/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 31503281 Number of paired-end alignments with a unique best hit: 16377060 Mapping efficiency: 52.0% Sequence pairs with no alignments under any condition: 8772590 Sequence pairs did not map uniquely: 6353631 Sequence pairs which were discarded because genomic sequence could not be extracted: 5 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4233791 ((converted) top strand) GA/CT/CT: 3962037 (complementary to (converted) top strand) GA/CT/GA: 3951534 (complementary to (converted) bottom strand) CT/GA/GA: 4229693 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 427057721 Total methylated C's in CpG context: 36699636 Total methylated C's in CHG context: 2370188 Total methylated C's in CHH context: 12902291 Total methylated C's in Unknown context: 1275308 Total unmethylated C's in CpG context: 17105060 Total unmethylated C's in CHG context: 88534839 Total unmethylated C's in CHH context: 269445707 Total unmethylated C's in Unknown context: 5454992 C methylated in CpG context: 68.2% C methylated in CHG context: 2.6% C methylated in CHH context: 4.6% C methylated in unknown context (CN or CHN): 18.9% Bismark completed in 0d 11h 56m 52s