Processing paired-end Bismark output file(s) (SAM format): zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_10_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam: 9282636 Total number duplicated alignments removed: 323003 (3.48%) Duplicated alignments were found at: 273923 different position(s) Total count of deduplicated leftover sequences: 8959633 (96.52% of total) Checking file >>zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_1_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam: 8273828 Total number duplicated alignments removed: 995382 (12.03%) Duplicated alignments were found at: 326089 different position(s) Total count of deduplicated leftover sequences: 7278446 (87.97% of total) Checking file >>zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_2_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam: 15247790 Total number duplicated alignments removed: 1127798 (7.40%) Duplicated alignments were found at: 674697 different position(s) Total count of deduplicated leftover sequences: 14119992 (92.60% of total) Checking file >>zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_3_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam: 15542347 Total number duplicated alignments removed: 734845 (4.73%) Duplicated alignments were found at: 572664 different position(s) Total count of deduplicated leftover sequences: 14807502 (95.27% of total) Checking file >>zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_4_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam: 12995899 Total number duplicated alignments removed: 332717 (2.56%) Duplicated alignments were found at: 284466 different position(s) Total count of deduplicated leftover sequences: 12663182 (97.44% of total) Checking file >>zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_5_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam: 16377055 Total number duplicated alignments removed: 841786 (5.14%) Duplicated alignments were found at: 694983 different position(s) Total count of deduplicated leftover sequences: 15535269 (94.86% of total) Checking file >>zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_6_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam: 13016029 Total number duplicated alignments removed: 367348 (2.82%) Duplicated alignments were found at: 309619 different position(s) Total count of deduplicated leftover sequences: 12648681 (97.18% of total) Checking file >>zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_7_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam: 15123226 Total number duplicated alignments removed: 505812 (3.34%) Duplicated alignments were found at: 440344 different position(s) Total count of deduplicated leftover sequences: 14617414 (96.66% of total) Checking file >>zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_8_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam: 14200405 Total number duplicated alignments removed: 747564 (5.26%) Duplicated alignments were found at: 527675 different position(s) Total count of deduplicated leftover sequences: 13452841 (94.74% of total) Checking file >>zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_9_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam: 16108083 Total number duplicated alignments removed: 2024669 (12.57%) Duplicated alignments were found at: 1660011 different position(s) Total count of deduplicated leftover sequences: 14083414 (87.43% of total)