Processing paired-end Bismark output file(s) (SAM format): zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_10_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam: 5299 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 5299 (100.00% of total) Checking file >>zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_1_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam: 2878 Total number duplicated alignments removed: 6 (0.21%) Duplicated alignments were found at: 6 different position(s) Total count of deduplicated leftover sequences: 2872 (99.79% of total) Checking file >>zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_2_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam: 4978 Total number duplicated alignments removed: 2 (0.04%) Duplicated alignments were found at: 2 different position(s) Total count of deduplicated leftover sequences: 4976 (99.96% of total) Checking file >>zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_3_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam: 5359 Total number duplicated alignments removed: 1 (0.02%) Duplicated alignments were found at: 1 different position(s) Total count of deduplicated leftover sequences: 5358 (99.98% of total) Checking file >>zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_4_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam: 5293 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 5293 (100.00% of total) Checking file >>zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_5_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam: 5173 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 5173 (100.00% of total) Checking file >>zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_6_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam: 5375 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 5375 (100.00% of total) Checking file >>zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_7_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam: 5144 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 5144 (100.00% of total) Checking file >>zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_8_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam: 4760 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 4760 (100.00% of total) Checking file >>zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_9_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam: 4994 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 4994 (100.00% of total)