Processing paired-end Bismark output file(s) (SAM format): zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_10_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam: 2085 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 2085 (100.00% of total) Checking file >>zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_1_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam: 816 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 816 (100.00% of total) Checking file >>zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_2_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam: 1915 Total number duplicated alignments removed: 1 (0.05%) Duplicated alignments were found at: 1 different position(s) Total count of deduplicated leftover sequences: 1914 (99.95% of total) Checking file >>zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_3_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam: 2144 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 2144 (100.00% of total) Checking file >>zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_4_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam: 2104 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 2104 (100.00% of total) Checking file >>zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_5_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam: 1996 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 1996 (100.00% of total) Checking file >>zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_6_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam: 2184 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 2184 (100.00% of total) Checking file >>zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_7_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam: 1930 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 1930 (100.00% of total) Checking file >>zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_8_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam: 1802 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 1802 (100.00% of total) Checking file >>zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_9_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam: 1890 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 1890 (100.00% of total)