Bismark report for: zr2096_5_s1_R1_val_1.fq.gz and zr2096_5_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/sr320/Dropbox/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 1000 Number of paired-end alignments with a unique best hit: 182 Mapping efficiency: 18.2% Sequence pairs with no alignments under any condition: 722 Sequence pairs did not map uniquely: 96 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 43 ((converted) top strand) GA/CT/CT: 46 (complementary to (converted) top strand) GA/CT/GA: 44 (complementary to (converted) bottom strand) CT/GA/GA: 49 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 4989 Total methylated C's in CpG context: 525 Total methylated C's in CHG context: 18 Total methylated C's in CHH context: 35 Total methylated C's in Unknown context: 0 Total unmethylated C's in CpG context: 126 Total unmethylated C's in CHG context: 1129 Total unmethylated C's in CHH context: 3156 Total unmethylated C's in Unknown context: 0 C methylated in CpG context: 80.6% C methylated in CHG context: 1.6% C methylated in CHH context: 1.1% Can't determine percentage of methylated Cs in unknown context (CN or CHN) if value was 0 Bismark completed in 0d 0h 0m 19s