Bismark report for: zr2096_4_s1_R1_val_1.fq.gz and zr2096_4_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/sr320/Dropbox/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 1000 Number of paired-end alignments with a unique best hit: 195 Mapping efficiency: 19.5% Sequence pairs with no alignments under any condition: 676 Sequence pairs did not map uniquely: 129 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 47 ((converted) top strand) GA/CT/CT: 49 (complementary to (converted) top strand) GA/CT/GA: 50 (complementary to (converted) bottom strand) CT/GA/GA: 49 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 5082 Total methylated C's in CpG context: 512 Total methylated C's in CHG context: 23 Total methylated C's in CHH context: 46 Total methylated C's in Unknown context: 0 Total unmethylated C's in CpG context: 174 Total unmethylated C's in CHG context: 1100 Total unmethylated C's in CHH context: 3227 Total unmethylated C's in Unknown context: 2 C methylated in CpG context: 74.6% C methylated in CHG context: 2.0% C methylated in CHH context: 1.4% C methylated in unknown context (CN or CHN): 0.0% Bismark completed in 0d 0h 0m 18s