Processing paired-end Bismark output file(s) (SAM format): zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_10_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam: 3585012 Total number duplicated alignments removed: 150670 (4.20%) Duplicated alignments were found at: 126749 different position(s) Total count of deduplicated leftover sequences: 3434342 (95.80% of total) Checking file >>zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_1_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam: 2299993 Total number duplicated alignments removed: 73049 (3.18%) Duplicated alignments were found at: 58714 different position(s) Total count of deduplicated leftover sequences: 2226944 (96.82% of total) Checking file >>zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_2_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam: 5898473 Total number duplicated alignments removed: 357487 (6.06%) Duplicated alignments were found at: 289095 different position(s) Total count of deduplicated leftover sequences: 5540986 (93.94% of total) Checking file >>zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_3_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam: 6143399 Total number duplicated alignments removed: 356192 (5.80%) Duplicated alignments were found at: 275036 different position(s) Total count of deduplicated leftover sequences: 5787207 (94.20% of total) Checking file >>zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_4_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam: 5194561 Total number duplicated alignments removed: 168028 (3.23%) Duplicated alignments were found at: 142341 different position(s) Total count of deduplicated leftover sequences: 5026533 (96.77% of total) Checking file >>zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_5_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam: 6213355 Total number duplicated alignments removed: 373151 (6.01%) Duplicated alignments were found at: 312001 different position(s) Total count of deduplicated leftover sequences: 5840204 (93.99% of total) Checking file >>zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_6_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam: 5127457 Total number duplicated alignments removed: 186706 (3.64%) Duplicated alignments were found at: 155273 different position(s) Total count of deduplicated leftover sequences: 4940751 (96.36% of total) Checking file >>zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_7_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam: 5560803 Total number duplicated alignments removed: 222796 (4.01%) Duplicated alignments were found at: 193251 different position(s) Total count of deduplicated leftover sequences: 5338007 (95.99% of total) Checking file >>zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_8_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam: 5162019 Total number duplicated alignments removed: 253566 (4.91%) Duplicated alignments were found at: 215890 different position(s) Total count of deduplicated leftover sequences: 4908453 (95.09% of total) Checking file >>zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: zr2096_9_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam [samopen] SAM header is present: 11 sequences. Total number of alignments analysed in zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam: 6030681 Total number duplicated alignments removed: 823325 (13.65%) Duplicated alignments were found at: 669678 different position(s) Total count of deduplicated leftover sequences: 5207356 (86.35% of total)