Bismark report for: zr2096_5_s1_R1.fastq.gz and zr2096_5_s1_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-virginica-oa/analyses/2018-04-27-Bismark/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 5290 Mapping efficiency: 52.9% Sequence pairs with no alignments under any condition: 3067 Sequence pairs did not map uniquely: 1643 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1343 ((converted) top strand) GA/CT/CT: 1308 (complementary to (converted) top strand) GA/CT/GA: 1287 (complementary to (converted) bottom strand) CT/GA/GA: 1352 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 176153 Total methylated C's in CpG context: 15197 Total methylated C's in CHG context: 1267 Total methylated C's in CHH context: 4823 Total methylated C's in Unknown context: 673 Total unmethylated C's in CpG context: 7270 Total unmethylated C's in CHG context: 37436 Total unmethylated C's in CHH context: 110160 Total unmethylated C's in Unknown context: 2631 C methylated in CpG context: 67.6% C methylated in CHG context: 3.3% C methylated in CHH context: 4.2% C methylated in unknown context (CN or CHN): 20.4% Bismark completed in 0d 0h 0m 24s