Bismark

Bismark Processing Report

zr2096_5_s1_R1.fastq.gz and zr2096_5_s1_R2.fastq.gz

Data processed at 17:36 on 2018-05-08


Alignment

Sequence pairs analysed in total 10,000
Paired-end alignments with a unique best hit 5,290
Pairs without alignments under any condition 3,067
Pairs that did not map uniquely 1,643
Genomic sequence context not extractable (edges of chromosomes) 0
Created with Highcharts 5.0.5: 52.9 %Unique AlignmentsUnique Alignments: 52.9 %: 30.7 %No AlignmentNo Alignment: 30.7 %: 16.4 %Multiple AlignmentsMultiple Alignments: 16.4 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 176,153
Methylated C's in CpG context 15,197
Methylated C's in CHG context 1,267
Methylated C's in CHH context 4,823
Methylated C's in Unknown context 673
Unmethylated C's in CpG context 7,270
Unmethylated C's in CHG context 37,436
Unmethylated C's in CHH context 110,160
Unmethylated C's in Unknown context 2,631
Percentage methylation (CpG context) 67.6%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 4.2%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 1,343 original top strand
CTOT 1,308 complementary to original top strand
CTOB 1,287 complementary to original bottom strand
OB 1,352 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB0250500750100012501500

Deduplication

Alignments analysed 5,290
Unique alignments 5,290
Duplicates removed 0
Duplicated alignments were found at 0 different positions
Created with Highcharts 5.0.5: 100.0 %UniqueUnique: 100.0 %: 0.0 %DuplicateDuplicate: 0.0 %