Bismark

Bismark Processing Report

/Volumes/web/nightingales/C_virginica/zr2096_3_s1_R1.fastq.gz

Data processed at 19:13 on 2018-04-29


Alignment

Sequences analysed in total 10,000
Single-end alignments with a unique best hit 6,272
Sequences without alignments under any condition 1,237
Sequences that did not map uniquely 2,491
Genomic sequence context not extractable (edges of chromosomes) 0
Created with Highcharts 5.0.5: 62.7 %Unique AlignmentsUnique Alignments: 62.7 %: 12.4 %No AlignmentNo Alignment: 12.4 %: 24.9 %Multiple AlignmentsMultiple Alignments: 24.9 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 109,168
Methylated C's in CpG context 12,011
Methylated C's in CHG context 784
Methylated C's in CHH context 2,675
Methylated C's in Unknown context 400
Unmethylated C's in CpG context 3,734
Unmethylated C's in CHG context 24,189
Unmethylated C's in CHH context 65,775
Unmethylated C's in Unknown context 1,825
Percentage methylation (CpG context) 76.3%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 3.9%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 1,401 original top strand
CTOT 1,666 complementary to original top strand
CTOB 1,722 complementary to original bottom strand
OB 1,483 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB0500100015002000