[2015-02-23 15:38:55] Beginning TopHat run (v2.0.13) ----------------------------------------------- [2015-02-23 15:38:55] Checking for Bowtie Bowtie version: 2.1.0.0 [2015-02-23 15:38:56] Checking for Bowtie index files (genome).. [2015-02-23 15:38:56] Checking for reference FASTA file [2015-02-23 15:38:56] Generating SAM header for /Volumes/web/halfshell/qdod3/Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel [2015-02-23 15:39:08] Preparing reads left reads: min. length=101, max. length=101, 28690571 kept reads (163825 discarded) [2015-02-23 15:59:45] Mapping left_kept_reads to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 [2015-02-23 16:53:43] Mapping left_kept_reads_seg1 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (1/4) [2015-02-23 17:00:06] Mapping left_kept_reads_seg2 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (2/4) [2015-02-23 17:06:25] Mapping left_kept_reads_seg3 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (3/4) [2015-02-23 17:12:42] Mapping left_kept_reads_seg4 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (4/4) [2015-02-23 17:21:18] Searching for junctions via segment mapping [2015-02-23 17:28:26] Retrieving sequences for splices [2015-02-23 17:29:03] Indexing splices [2015-02-23 17:31:03] Mapping left_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/4) [2015-02-23 17:34:16] Mapping left_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/4) [2015-02-23 17:37:38] Mapping left_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/4) [2015-02-23 17:40:58] Mapping left_kept_reads_seg4 to genome segment_juncs with Bowtie2 (4/4) [2015-02-23 17:44:49] Joining segment hits [2015-02-23 17:52:18] Reporting output tracks ----------------------------------------------- [2015-02-23 18:11:36] A summary of the alignment counts can be found in ./tophat_out/align_summary.txt [2015-02-23 18:11:36] Run complete: 02:32:40 elapsed