[2015-02-20 15:41:30] Beginning TopHat run (v2.0.13) ----------------------------------------------- [2015-02-20 15:41:30] Checking for Bowtie Bowtie version: 2.1.0.0 [2015-02-20 15:41:30] Checking for Bowtie index files (genome).. [2015-02-20 15:41:30] Checking for reference FASTA file [2015-02-20 15:41:30] Generating SAM header for /Volumes/web/halfshell/qdod3/Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel [2015-02-20 15:41:32] Preparing reads left reads: min. length=101, max. length=101, 23807053 kept reads (88171 discarded) [2015-02-20 16:00:09] Mapping left_kept_reads to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 [2015-02-20 16:50:34] Mapping left_kept_reads_seg1 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (1/4) [2015-02-20 16:56:59] Mapping left_kept_reads_seg2 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (2/4) [2015-02-20 17:03:24] Mapping left_kept_reads_seg3 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (3/4) [2015-02-20 17:09:56] Mapping left_kept_reads_seg4 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (4/4) [2015-02-20 17:18:11] Searching for junctions via segment mapping [2015-02-20 17:25:13] Retrieving sequences for splices [2015-02-20 17:25:52] Indexing splices [2015-02-20 17:27:43] Mapping left_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/4) [2015-02-20 17:31:19] Mapping left_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/4) [2015-02-20 17:35:08] Mapping left_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/4) [2015-02-20 17:38:49] Mapping left_kept_reads_seg4 to genome segment_juncs with Bowtie2 (4/4) [2015-02-20 17:42:56] Joining segment hits [2015-02-20 17:50:33] Reporting output tracks ----------------------------------------------- [2015-02-20 18:07:39] A summary of the alignment counts can be found in 4M/align_summary.txt [2015-02-20 18:07:39] Run complete: 02:26:08 elapsed