[2015-02-20 18:07:40] Beginning TopHat run (v2.0.13) ----------------------------------------------- [2015-02-20 18:07:40] Checking for Bowtie Bowtie version: 2.1.0.0 [2015-02-20 18:07:41] Checking for Bowtie index files (genome).. [2015-02-20 18:07:41] Checking for reference FASTA file [2015-02-20 18:07:41] Generating SAM header for /Volumes/web/halfshell/qdod3/Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel [2015-02-20 18:07:54] Preparing reads left reads: min. length=101, max. length=101, 18308054 kept reads (479804 discarded) [2015-02-20 18:22:17] Mapping left_kept_reads to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 [2015-02-20 19:10:34] Mapping left_kept_reads_seg1 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (1/4) [2015-02-20 19:16:38] Mapping left_kept_reads_seg2 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (2/4) [2015-02-20 19:22:20] Mapping left_kept_reads_seg3 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (3/4) [2015-02-20 19:28:11] Mapping left_kept_reads_seg4 to genome Crassostrea_gigas.GCA_000297895.1.25.dna_sm.toplevel with Bowtie2 (4/4) [2015-02-20 19:35:23] Searching for junctions via segment mapping [2015-02-20 19:40:57] Retrieving sequences for splices [2015-02-20 19:41:32] Indexing splices [2015-02-20 19:42:46] Mapping left_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/4) [2015-02-20 19:45:27] Mapping left_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/4) [2015-02-20 19:48:10] Mapping left_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/4) [2015-02-20 19:50:49] Mapping left_kept_reads_seg4 to genome segment_juncs with Bowtie2 (4/4) [2015-02-20 19:53:28] Joining segment hits [2015-02-20 19:59:41] Reporting output tracks ----------------------------------------------- [2015-02-20 20:13:34] A summary of the alignment counts can be found in 4M-HS/align_summary.txt [2015-02-20 20:13:34] Run complete: 02:05:53 elapsed