Bismark report for: /Volumes/Serine/wd/18-04-27/zr2096_3_s1_R1_val_1.fq.gz and /Volumes/Serine/wd/18-04-27/zr2096_3_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-0.9 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 29548753 Number of paired-end alignments with a unique best hit: 13093747 Mapping efficiency: 44.3% Sequence pairs with no alignments under any condition: 10404990 Sequence pairs did not map uniquely: 6050016 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3279278 ((converted) top strand) GA/CT/CT: 3253127 (complementary to (converted) top strand) GA/CT/GA: 3268922 (complementary to (converted) bottom strand) CT/GA/GA: 3292419 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 364333091 Total methylated C's in CpG context: 42231693 Total methylated C's in CHG context: 1748101 Total methylated C's in CHH context: 6570371 Total methylated C's in Unknown context: 473894 Total unmethylated C's in CpG context: 11323986 Total unmethylated C's in CHG context: 81087161 Total unmethylated C's in CHH context: 221371779 Total unmethylated C's in Unknown context: 2480520 C methylated in CpG context: 78.9% C methylated in CHG context: 2.1% C methylated in CHH context: 2.9% C methylated in unknown context (CN or CHN): 16.0% Bismark completed in 0d 4h 29m 43s