Bismark report for: /Volumes/Serine/wd/18-04-10/trim14/zr2096_6_s1_R1_val_1.fq.gz and /Volumes/Serine/wd/18-04-10/trim14/zr2096_6_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 24080119 Number of paired-end alignments with a unique best hit: 13889795 Mapping efficiency: 57.7% Sequence pairs with no alignments under any condition: 5415629 Sequence pairs did not map uniquely: 4774695 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3653608 ((converted) top strand) GA/CT/CT: 3286595 (complementary to (converted) top strand) GA/CT/GA: 3290365 (complementary to (converted) bottom strand) CT/GA/GA: 3659227 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 380247018 Total methylated C's in CpG context: 36675468 Total methylated C's in CHG context: 2049817 Total methylated C's in CHH context: 9632476 Total methylated C's in Unknown context: 1019260 Total unmethylated C's in CpG context: 13557194 Total unmethylated C's in CHG context: 81881435 Total unmethylated C's in CHH context: 236450628 Total unmethylated C's in Unknown context: 5065365 C methylated in CpG context: 73.0% C methylated in CHG context: 2.4% C methylated in CHH context: 3.9% C methylated in unknown context (CN or CHN): 16.8% Bismark completed in 0d 4h 19m 45s