FastQCFastQC Report
Tue 10 Apr 2018
2112_lane1_NoIndex_L001_R1_012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_NoIndex_L001_R1_012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4937833.08614375TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3972992.48311875TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3124281.952675TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2654151.6588437500000002TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2461641.538525TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2183531.36470625TruSeq Adapter, Index 6 (100% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC278680.174175TruSeq Adapter, Index 7 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC243090.15193125000000002TruSeq Adapter, Index 4 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC231720.144825TruSeq Adapter, Index 7 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC225710.14106875TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT211790.13236875TruSeq Adapter, Index 7 (100% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC184870.11554375TruSeq Adapter, Index 5 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC179650.11228124999999999TruSeq Adapter, Index 4 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC177670.11104375000000001TruSeq Adapter, Index 4 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT164570.10285625TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGA224900.085.280161
CCGGAAG197400.084.895153
GACCGGA170900.083.1894451
ACCGGAA207750.081.2844242
GAGCACA4301500.075.261579
AGAGCAC4325900.074.940468
GAAGAGC4385600.074.40376
AAGAGCA4398050.074.067047
GGAAGAG4432150.072.9687965
GATTGGA170450.072.784251
ATTGGAA182000.072.415512
ACGGAAG118750.072.32233
CGGAAGA4288150.072.218874
TTGGAAG184900.071.3559653
TCGGAAG3942300.071.330893
TGGAAGA205800.070.67944
ATCGGAA4029950.069.9136052
AGCGGAA93550.069.095252
GCGGAAG92400.068.874163
GAGCGGA81100.068.4566961
Produced by FastQC (version 0.11.5)