FastQCFastQC Report
Tue 10 Apr 2018
2112_lane1_NoIndex_L001_R1_011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_NoIndex_L001_R1_011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4919343.0745875TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3921282.4507999999999996TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3111321.9445750000000002TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2665531.6659562499999998TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2462151.5388437499999998TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2150601.344125TruSeq Adapter, Index 6 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC537000.335625TruSeq Adapter, Index 7 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC421720.263575TruSeq Adapter, Index 4 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC312530.19533125TruSeq Adapter, Index 5 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC299180.1869875TruSeq Adapter, Index 1 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC293390.18336875TruSeq Adapter, Index 7 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC278550.17409375000000002TruSeq Adapter, Index 3 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT271590.16974375TruSeq Adapter, Index 7 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC246410.15400625TruSeq Adapter, Index 6 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC226190.14136875TruSeq Adapter, Index 4 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC220990.13811875TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT215790.13486875TruSeq Adapter, Index 4 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT189750.11859375TruSeq Adapter, Index 5 (100% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC171270.10704375TruSeq Adapter, Index 4 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC171130.10695625TruSeq Adapter, Index 5 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT163000.101875TruSeq Adapter, Index 1 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC160290.10018125000000001TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAG324400.089.272953
GACCGGA319750.088.9973141
ACCGGAA337950.086.1882
ACGGAAG153600.077.600623
GATTGGA196900.077.02831
ATTGGAA201000.076.4857252
CGAAGAG111900.076.012815
TTGGAAG202600.075.7623143
TGGAAGA233900.075.031244
GAGCACA4198000.074.378739
AGAGCAC4226400.073.929518
GAAGAGC4266300.073.564116
AAGAGCA4284400.073.161327
TCCGAAG83100.072.889483
GGAAGAG4287950.071.899085
CGGAAGA4133600.070.6983644
GAACGGA165950.070.292241
TCGGAAG3620250.069.158293
ATCGGAA3704700.067.7927252
GATCGGA3734100.066.215471
Produced by FastQC (version 0.11.5)