FastQCFastQC Report
Tue 10 Apr 2018
2112_lane1_NoIndex_L001_R1_007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_NoIndex_L001_R1_007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3697012.31063125TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3178881.9868000000000001TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2434881.5218TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2008971.25560625TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1886351.17896875TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1706991.06686875TruSeq Adapter, Index 6 (100% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC447090.27943125TruSeq Adapter, Index 7 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC262070.16379375000000002TruSeq Adapter, Index 7 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC215690.13480625000000002TruSeq Adapter, Index 1 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC214980.1343625TruSeq Adapter, Index 5 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC211140.1319625TruSeq Adapter, Index 4 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC196770.12298125TruSeq Adapter, Index 7 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC195400.12212499999999998TruSeq Adapter, Index 6 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC179410.11213124999999999TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAG365250.089.868093
CGAAGAG434250.089.425195
CCGAAGA449600.086.44384
CCGGAAG232600.086.207723
GATCCGA321300.085.99361
GACCGGA216850.085.461471
CATCGGA197650.082.9416961
ACCGGAA249100.082.6753852
GCGGAAG142350.079.277433
AGCGGAA146150.078.552352
GAGCGGA136000.077.202851
AGAGCAC4275300.076.760118
GAGCACA4289200.076.479719
GAAGAGC4308700.076.3726046
AAGAGCA4329850.075.8634647
ACGGAAG128100.073.4445343
GGAAGAG3910200.072.616495
CGGAAGA3883800.072.150364
TCGGAAG3504000.069.395083
ATCGGAA3591750.068.053032
Produced by FastQC (version 0.11.5)