FastQCFastQC Report
Tue 10 Apr 2018
2112_lane1_NoIndex_L001_R1_006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_NoIndex_L001_R1_006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4930333.08145625TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3906362.441475TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3090911.9318187500000001TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2798031.7487687500000002TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2454461.5340375TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2150171.34385625TruSeq Adapter, Index 6 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC300730.18795625TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT278290.17393125TruSeq Adapter, Index 7 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC232770.14548125TruSeq Adapter, Index 4 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT221410.13838125TruSeq Adapter, Index 4 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT195740.1223375TruSeq Adapter, Index 5 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT174460.1090375TruSeq Adapter, Index 1 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC172910.10806875TruSeq Adapter, Index 5 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC167470.10466875TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA210450.085.1362841
CCGGAAG214350.084.592763
ACCGGAA224550.081.988172
CGAAGAG133150.078.037345
TCCGAAG109300.077.777433
GAGCACA4006100.073.618779
AGAGCAC4035900.073.043498
GAAGAGC4094800.072.140966
AAGAGCA4100050.072.005637
GATCCGA123000.070.746591
GGAAGAG4085050.070.457765
CGGAAGA4035850.069.7607654
TCGGAAG3678350.069.474053
CCGAAGA149850.069.338974
ATCGGAA3761150.068.145912
ACGGAAG99050.067.174493
GATCGGA3819350.066.920891
GATTGGA103150.061.3701671
GAGCGGA68350.059.727851
AATCATA1235950.059.0029145
Produced by FastQC (version 0.11.5)