FastQCFastQC Report
Tue 10 Apr 2018
2112_lane1_NoIndex_L001_R1_005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_NoIndex_L001_R1_005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4589692.86855625TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3757162.348225TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2897421.8108875TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2597431.62339375TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2273311.42081875TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2026831.26676875TruSeq Adapter, Index 6 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC200800.1255TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT200350.12521875000000002TruSeq Adapter, Index 7 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC164990.10311875TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAG187550.085.7274553
CCGGAAG192250.084.990913
CGAAGAG238250.084.730425
GACCGGA171400.082.4472661
ACCGGAA206950.081.412452
CCGAAGA252900.080.162194
CATCGGA126700.078.322061
GATCCGA165100.077.414171
GAGCACA4135650.075.386739
AGAGCAC4141100.075.266138
GAAGAGC4195200.074.373526
AAGAGCA4194300.074.275937
GGAAGAG4036600.071.938595
CGGAAGA3997100.071.299454
TCGGAAG3704250.070.445413
ATCGGAA3791500.069.146862
GATCGGA3759800.067.2115861
ACGGAAG99600.066.1643143
AGCGGAA79500.063.172112
GCGGAAG77850.062.8615763
Produced by FastQC (version 0.11.5)