FastQCFastQC Report
Tue 10 Apr 2018
2112_lane1_ATCACG_L001_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_ATCACG_L001_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409414
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1119277.9413855687541055TruSeq Adapter, Index 1 (100% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC84030.5962052313940404TruSeq Adapter, Index 1 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC75490.5356126730683817TruSeq Adapter, Index 1 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT49250.34943600673755193TruSeq Adapter, Index 1 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC41080.2914686529295154TruSeq Adapter, Index 1 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC40220.28536682621287995TruSeq Adapter, Index 1 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC33690.2390355140505203TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGATCTCGTATGC19220.1363687319694568TruSeq Adapter, Index 1 (98% over 50bp)
CGCAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT15510.11004573531978537TruSeq Adapter, Index 1 (98% over 50bp)
CGTAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT15500.10997478384633613TruSeq Adapter, Index 1 (98% over 50bp)
CACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC15490.1099038323728869TruSeq Adapter, Index 1 (100% over 47bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC14620.10373105418280222TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGA10700.085.685221
CCGGAAG12500.083.9874953
TCTGAAG17550.082.015873
CTGAAGA19750.081.776544
GACCGGA10300.079.788681
ACCGGAA12950.078.868052
TGAAGAG22050.072.8027955
GAGCACA249850.072.291449
AGAGCAC250900.072.0267648
GAAGAGC252600.071.542036
AAGAGCA255550.070.7533347
AGCGGAA8300.068.680832
GCGGAAG8300.068.680833
GAGCGGA7300.068.328011
TAAGAGC4600.068.158263
GGAAGAG243350.067.762325
CGGAAGA236800.067.402274
ACGGAAG8800.066.397983
CTCTGAA10800.065.538262
ATCTGAA11250.065.028032
Produced by FastQC (version 0.11.5)