FastQCFastQC Report
Sat 7 Apr 2018
zr2096_9_s1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_9_s1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32636231
Sequences flagged as poor quality0
Sequence length20-100
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT23500.079.1664051
TGGCACC10650.073.812224
CTTGGCA11600.073.238752
CCTTGTG70950.072.862197
ACCCTTG71550.072.6425555
TTGGCAC11350.072.244273
CCTTGGC11850.072.0757451
CTGTGCT14100.071.069841
CCCTTGT75100.069.978866
CCGTGCT12650.069.523071
GGCACCC10900.069.006365
GCCTGAG2550.066.32481
CAGTGCT10650.066.301451
GGCTGCT9700.066.2564241
GCCTGGT5950.066.087931
TGTGCTC53450.066.030052
GCGTGCT8700.065.610091
GACTGCT6350.065.25421
GCTGGCT5150.064.8598561
CCTGGCT5900.063.7814061
Produced by FastQC (version 0.11.5)