FastQCFastQC Report
Sat 7 Apr 2018
zr2096_7_s1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_7_s1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29534746
Sequences flagged as poor quality0
Sequence length20-100
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCT5100.080.801531
GCCTGCT19200.080.038761
TGCAGCT5700.079.073871
GGCAGCT2100.077.6752851
CCTGGCT6550.077.3321
CTGTGCT7500.075.549431
GCCTGAT7700.074.1445851
GCCTGGG4300.073.872331
GTCAGCT5850.072.6436841
GCCTGGT5750.072.413981
GCCAGGG950.072.2961041
CCGTGCT8450.072.135681
GTCTGCT7300.070.562981
GCATGCT2200.070.242241
CCAGGCT6550.070.1230851
GCTAGCT4400.069.266651
GGGCCAA258.71517E-568.68131
GCCTGGA2950.068.3902741
CATGGCT3800.067.7775961
ACTGGCT5250.067.046031
Produced by FastQC (version 0.11.5)